Procedures

Day 1:
  1. Use a cotton swab to take samples from a tray from a canteen and inoculate in LB tubes.
  2. Incubate the tube at 37 degrees celsius with shaking(220 rpm) for 1 day. Remember to label the tubes.
Day 2:
  1. Prepare a selective agar plates and label these plates
  2. Choose 1 selective plate.
  3. Take the inoculating loop and hold it like a pencil. Use sterile disposable inoculating loop.
  4. Open the lid of the Petri dish culture to a gap and cool the hot loop by inserting it into the agar without touching any colonies developed on the surface. Choose a discrete colony and pick a loopful of inoculum using the inoculating loop, and then close the lid of the Petri dish.
  5. Using the same hand that is holding the inoculating loop, remove e the cap from a test tube, hold it between your fingers, and briefly flame the neck of the tube over the methylated spirit lamp by passing through the lamp.
  6. Inoculate the surface of the agar slant in zigzag streaks using the infected inoculating loop
  7. Flame the neck if the tube again and close it with the cap.
  8. Choose another selective plate and repeat 3-8.
  9. Use new sterile disposable inoculating loop.
  10. Place the tube and the inoculating loop on the rack.
  11. Incubate the the slant at 37 degrees celsius for 1 day.
  12. Check the growth of the isolate after the incubation period.

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